polyclonal sheep anti lyve1  (R&D Systems)

Journal: Cells

Article Title: Single-Cell RNA-Seq Reveals a Crosstalk between Hyaluronan Receptor LYVE-1-Expressing Macrophages and Vascular Smooth Muscle Cells

doi: 10.3390/cells11030411

Figure Lengend Snippet: LYVE-1 res-like macrophages in mouse atherosclerotic plaques. Bar graphs represent the mean ± SEM of the percentage of LYVE1 res-like macrophages in AA&R of Apoe −/− mice on NCD versus HCD, expressed as percentage of the total live plaque cells, as defined by ( A ) LYVE1 + CD64 + ; ( B ) LYVE1 + CD64 + CD163 + ; ( C ) LYVE1 + CD64 + CCL24 + ; and ( D ) LYVE1 + CD64 + CD115 + , with an expression of n = 8 mice per group and *** p < 0.001. Representative dot plots show the gating strategy illustrating LYVE1 + res-like macrophage marker expression in AA&R of Apoe −/− mice on ( E ) NCD and ( F ) HCD. Bar graphs represent the mean ± SEM of the percentage of LYVE1 res-like macrophages in abdominal aortas of Apoe −/− mice on NCD versus HCD, expressed as percentage of the total live plaque cells, as defined by ( G ) LYVE1 + CD64 + ; ( H ) LYVE1 + CD64 + CD163 + ; ( I ) LYVE1 + CD64 + CCL24 + ; and ( J ) LYVE1 + CD64 + CD115 + , with an expression of n = 8 mice per group and *** p < 0.001. Representative dot plots illustrating the gating strategy show the LYVE1 + res-like macrophage marker expression in abdominal aortas of Apoe −/− mice on ( K ) NCD and ( L ) HCD.

Article Snippet: Endarterectomy specimens were stained with a primary rat anti-LYVE1 (R&D Systems, Minneapolis, MN, USA), an anti-Myh11 (Thermo Fischer, Waltham, MA, USA), and a goat anti-CCL24 in a blocking solution.

Techniques: Expressing, Marker

Journal: Cells

Article Title: Single-Cell RNA-Seq Reveals a Crosstalk between Hyaluronan Receptor LYVE-1-Expressing Macrophages and Vascular Smooth Muscle Cells

doi: 10.3390/cells11030411

Figure Lengend Snippet: The role of LYVE-1 res-like macrophages in vascular calcification. ( A ) Representative images of vascular calcification illustrated that Alizarin red positively stained areas in aortic root cryosections of Apoe −/− Myh11-CreERT2, ROSA26STOP-flox eYFP +/+ mice fed NCD or HCD. Scale bars: 200 μm. ( B ) Bar graphs represent the mean ± SEM of the percentage of Alizarin red positively stained areas in the total atherosclerotic plaque in aortic root cryosections of Apoe −/− Myh11-CreERT2, ROSA26STOP-flox eYFP +/+ mice fed NCD or HCD, n = 8 and ** p < 0.01. ( C ) Representative immunofluorescence staining showing LYVE1 + CCL24 + cells in close proximity to VSMC (Myh11 positive cells) in atherosclerotic lesions of Apoe −/− mice on HCD; Myh11 (green), LYVE1 (red), and CCL24 (purple). ( D ) Bar graphs represent the mean ± SEM of systemic levels of CCL24 in Apoe −/− mice on NCD and HCD, n = 8 mice per group and ** p < 0.01. Bar graph representing the mean ± SEM of ( E ) α-SMA + OPN + ; ( F ) α-SMA + RUNX2 + ; ( G ) α-SMA + alkaline phosphatase + ; ( H ) α-SMA + MAC2 + ; ( I ) α-SMA + F4/80 + expression in WT VSMC post-stimulation with oxLDL or co-cultured with LYVE-1 res-like macrophages in the presence or absence of CCL24 neutralizing antibodies, n = 6 and *** p < 0.001.

Article Snippet: Endarterectomy specimens were stained with a primary rat anti-LYVE1 (R&D Systems, Minneapolis, MN, USA), an anti-Myh11 (Thermo Fischer, Waltham, MA, USA), and a goat anti-CCL24 in a blocking solution.

Techniques: Staining, Immunofluorescence, Expressing, Cell Culture

Journal: Cells

Article Title: Single-Cell RNA-Seq Reveals a Crosstalk between Hyaluronan Receptor LYVE-1-Expressing Macrophages and Vascular Smooth Muscle Cells

doi: 10.3390/cells11030411

Figure Lengend Snippet: LYVE-1 res-like macrophages in human atherosclerotic plaques. ( A ) Representative images of vascular calcification illustrated by Alizarin red staining of human atherosclerotic plaques of asymptomatic and symptomatic CAD patients, respectively. Scale bars: 800 μm. ( B ) Bar graphs represent the mean ± SEM of the percentage of Alizarin red positively stained areas in the total atherosclerotic plaque area of asymptomatic and symptomatic CAD patients, respectively, n = 8 and * p < 0.05. ( C ) Representative immunofluorescence staining showing LYVE1 + cells (red) expressing CCL24 + (violet) in atherosclerotic lesions of asymptomatic and symptomatic CAD patients, respectively. ( D ) Bar graphs represent the mean ± SEM of LYVE1 cells expressing CCL24 in asymptomatic and symptomatic CAD patients, respectively, n = 8 and * p < 0.05. ( E ) Positive correlation of vascular calcification (percentage of positive Alizarin red stained areas) with percentage of LYVE1 cells expressing CCL24. ( F ) Bar graphs represent the mean ± SEM of systemic levels of CCL24 + in asymptomatic and symptomatic CAD patients, respectively, n = 8 and *** p < 0.001. ( G ) Positive correlation of vascular calcification (percentage of positive Alizarin red stained areas) with systemic levels of CCL24.

Article Snippet: Endarterectomy specimens were stained with a primary rat anti-LYVE1 (R&D Systems, Minneapolis, MN, USA), an anti-Myh11 (Thermo Fischer, Waltham, MA, USA), and a goat anti-CCL24 in a blocking solution.

Techniques: Staining, Immunofluorescence, Expressing